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The 18th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA 2019) is scheduled to be held from 24th to 26th of October, 2019 at the National Convention Center, Beijing China. In the same period of the exhibition, 10 academic sessions and forums in different fields were established. Each session and forum invited 10-50 scientists to share their latest research results, exchange and talk about cutting-edge technologies and new methods.
Dr. Weifei Zhang, a postdoctoral in our group, won the poster award with the title of " Inkjet Printing Based Droplet Generation for Integrated Online Digital Polymerase Chain Reaction ". This study reports on the development of a novel and flexible online digital polymerase chain reaction (dPCR) system. The system was composed of three parts: an inkjet for generating the droplets, a coiled fused-silica capillary for thermal cycling, and a laser-induced fluorescence detector (LIFD) for positive droplet counting. Upon inkjet printing, monodisperse droplets were continuously generated in the oil phase and then introduced into the capillary in the form of a stable dispersion. The droplets containing one or zero molecules of target DNA passed through the helical capillary that was attached to a cylindrical thermal cycler for PCR amplification, resulting in the generation of fluorescence for the DNA-positive droplet. After 36 PCR cycles, the fluorescence signal intensity was detected by laser-induced fluorescence located at the downstream of the capillary, followed by a positive/negative counting. In comparison with previous reports, our system was stable, flexible, and inexpensive, without liquid transfer steps eliminating material loss or cross-contamination. We believe that this homemade equipment has the potential to be widely applied in diverse areas, including biological and chemical research.